A Simple Approach to Improving RNA Synthesis: Salt Inhibition of RNA Rebinding Coupled With Strengthening Promoter Binding by a Targeted Gap in the DNA
Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, in press, 2023 Use this link for free access through August 26! T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large …