T7 RNA polymerase - Martin Lab

High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs

By Martin Posted on January 31, 2023 Posted in Publications, Uncategorized

Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, gkad027, https://doi.org/10.1093/nar/gkad027Published: 31 January 2023 T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science …

High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs Read more »

Tagged with: rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase

By Martin Posted on August 8, 2019 Posted in Publications

Yasaman Gholamalipour, William C. Johnson, & Craig T. Martin, Nucl Acids Res, 47, e118 (7 pages). 2019 In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as …

Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase Read more »

Tagged with: in vitro, RNA, rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

3’ end additions by T7 RNA polymerase are RNA self-templated, distributive, and diverse in character – RNA-Seq analyses

By Posted on September 28, 2018 Posted in Publications

Yasaman Gholamalipour, Aruni Karunanayake Mudiyanselage, & Craig T. Martin, Nucl Acids Res, 46, 9253-9263, 2018. Selected by the editors as a “Breakthrough Article.” Synthetic RNA is widely used in basic science, nanotechnology and therapeutics research. The vast majority of this RNA is …

3’ end additions by T7 RNA polymerase are RNA self-templated, distributive, and diverse in character – RNA-Seq analyses Read more »

Tagged with: in vitro, RNASeq, T7 RNA polymerase, transcription

Insights into the Mechanism of Initial Transcription in E coli RNA Polymerase. Energetic Mechanisms Leading to Initial Complex Instability and Abortive Cycling

By Posted on September 18, 2013 Posted in Publications

Satamita Samanta & Craig T. Martin, J. Biol. Chem. 288, 31993-32003, 2013. It has long been known that during initial transcription of the first 8-10 bases of RNA, complexes are relatively unstable, leading to the release of short abortive RNA transcripts. An …

Insights into the Mechanism of Initial Transcription in E coli RNA Polymerase. Energetic Mechanisms Leading to Initial Complex Instability and Abortive Cycling Read more »

Tagged with: abortive cycling, bacterial rna polymerase, in vitro, rna polymerase, T7 RNA polymerase, transcription

New Insights into the Mechanism of Initial Transcription: the T7 RNA Polymerase Mutant P266L Transitions to Elongation at Longer RNA Lengths than Wild Type

By Posted on May 30, 2012 Posted in Publications

Luis Ramírez-Tapia & Craig T. Martin, J. Biol. Chem. 287, 37352-37361, 2012. RNA polymerases undergo substantial structural and functional changes in transitioning from sequence-specific initial transcription to stable and relatively sequence-independent elongation. Initially transcribing complexes are characteristically unstable, yielding short abortive products …

New Insights into the Mechanism of Initial Transcription: the T7 RNA Polymerase Mutant P266L Transitions to Elongation at Longer RNA Lengths than Wild Type Read more »

Tagged with: abortive cycling, in vitro, P266L, rna polymerase, T7 RNA polymerase, transcription

Direct Tests of the Energetic Basis of Abortive Cycling in Transcription

By Posted on May 30, 2011 Posted in Publications

Ankit V. Vahia & Craig T. Martin, Biochemistry 50, 7015-7022, 2011. Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that …

Direct Tests of the Energetic Basis of Abortive Cycling in Transcription Read more »

Tagged with: abortive cycling, in vitro, initial transcription, initiation, rna polymerase, T7 RNA polymerase, transcription, transcription initiation

Transcription Elongation Complex Stability: The Topological Lock

By Posted on May 30, 2009 Posted in Publications

Xiaoqing Liu & Craig T. Martin, J. Biol. Chem. 284, 36262-36270, 2009. Transcription machinery from a variety of organisms shows striking mechanistic similarity. Both multi- and single subunit RNA polymerases have evolved an 8-10 base pair RNA:DNA hybrid as a part of …

Transcription Elongation Complex Stability: The Topological Lock Read more »

Tagged with: elongation, forward translocation, rna polymerase, stability, T7 RNA polymerase, topology

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