RNA synthesis - Martin Lab

High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs

By Martin Posted on January 31, 2023 Posted in Publications, Uncategorized

Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, gkad027, https://doi.org/10.1093/nar/gkad027Published: 31 January 2023 T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science …

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Tagged with: rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase

By Martin Posted on August 8, 2019 Posted in Publications

Yasaman Gholamalipour, William C. Johnson, & Craig T. Martin, Nucl Acids Res, 47, e118 (7 pages). 2019 In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as …

Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase Read more »

Tagged with: in vitro, RNA, rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

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High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs

Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase