High salt transcription of DNA co-tethered with T7 RNA polymerase to beads generates increased yields of highly pure RNA
Elvan Cavac, Luis E. Ramírez-Tapia, and Craig T. Martin, J Biol Chem, 297(3), 100999, 2021. doi: 10.1016/j.jbc.2021.100999 High yields of RNA are routinely prepared following the two-step approach of high-yield in vitro transcription using T7 RNA polymerase followed by extensive purification using gel separation …