Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, in press, 2023 Use this link for free access through August 26! T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large …

A Simple Approach to Improving RNA Synthesis: Salt Inhibition of RNA Rebinding Coupled With Strengthening Promoter Binding by a Targeted Gap in the DNA Read more »

Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, gkad027, https://doi.org/10.1093/nar/gkad027Published: 31 January 2023 T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science …

High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs Read more »

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Craig T. Martin and Joseph E. Coleman, Biochemistry 26, 2690-2696, 1987 Specific interactions between T7 RNA polymerase and its promoter have been studied by asimple steady-state kinetic assay using synthetic oligonucleotide promoters that produce a short five-basemessage. A series of …

Kinetic Analysis of T7 RNA Polymerase-Promoter Interactions with Small Synthetic Promoters Read more »