Kithmie MalagodaPathiranage, Ruptanu Banerjee, and Craig T. Martin, Nucleic Acids Research, July, 2024 Current approaches to RNA synthesis/manufacturing require substantial (and incomplete) purification post-synthesis. We have previously demonstrated the synthesis of RNA from a complex in which T7 RNA polymerase is …
Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, 691, 209-222, 2023 T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large amounts of RNA. Such synthesis, however, is often plagued …
Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, 51(6), e36 (12 pages) T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science to …
Craig T. Martin and Joseph E. Coleman, Biochemistry 26, 2690-2696, 1987 Specific interactions between T7 RNA polymerase and its promoter have been studied by asimple steady-state kinetic assay using synthetic oligonucleotide promoters that produce a short five-basemessage. A series of …