Novel RNA Manufacturing
Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, in press, 2023 Use this link for free access through August 26! T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large …
Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, gkad027, https://doi.org/10.1093/nar/gkad027Published: 31 January 2023 T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science …
Elvan Cavac, Luis E. Ramírez-Tapia, and Craig T. Martin, J Biol Chem, 297(3), 100999, 2021. doi: 10.1016/j.jbc.2021.100999 High yields of RNA are routinely prepared following the two-step approach of high-yield in vitro transcription using T7 RNA polymerase followed by extensive purification using gel separation …
Yasaman Gholamalipour, William C. Johnson, & Craig T. Martin, Nucl Acids Res, 47, e118 (7 pages). 2019 In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as …
Craig T. Martin & Karsten Theis, Nat Struct Mol Biol 21, 741–742, 2014. - 10.1038/nsmb.2883 PMID: 25192262 News & Views - Fluorescent probes at the exit channel and near the active site of RNA polymerase shed light on how transcriptional pausing is regulated.
C T Martin, C P Scholes, and S I Chan, Journal of Biological Chemistry 263, 8420-8429, 1988 The resolution of new features in the 1H electron nuclear double resonance (ENDOR) spectrum of the oxidized CuA site in beef heart cytochrome …
On the nature of cysteine coordination to CuA in cytochrome c oxidase Read more »
Craig T. Martin and Joseph E. Coleman, Biochemistry 26, 2690-2696, 1987 Specific interactions between T7 RNA polymerase and its promoter have been studied by asimple steady-state kinetic assay using synthetic oligonucleotide promoters that produce a short five-basemessage. A series of …
Craig T. Martin, Charles P. Scholes, Sunney I. Chan, Journal of Biological Chemistry 260, 2857-2861, 1985 A histidine auxotroph of Saccharomyces cerevisiae has been used to metabolically incorporate [1,3-15N2] histidine into yeast cytochrome c oxidase. Electron nuclear double resonance (ENDOR) …
The identification of histidine ligands to cytochrome a in cytochrome c oxidase Read more »
Robert R. Birge, Albert F. Lawrence, Thomas M. Cooper, Craig T. Martin, David F. Blair, Sunney I. Chan, Nonlinear Electrodynamics in Biological Systems, 107-120, 1984 The energy stored in the primary photochemical event of light adapted bacteriorhodopsin (~16 kcal mol-1) …
Sunney I. Chan, David F. Blair, Craig T. Martin, Hsin Wang, Jeff Gelles, JE Morgan, Steve Witt, RR Birge, Tom H. Stevens, Gary W. Brudvig, Inorganica Chimica Acta 79 72-73 1983 Considerable progress has been made in recent years on …
The metal centers of cytochrome c oxidase: Structure and function Read more »
DF Blair, GW Campbell, V Lum, CT Martin, HB Gray, BG Malmström, Sunney I. Chan, Journal of Inorganic Biochemistry 19, 65-73 Resonance Raman (RR) spectra of Rhus vernicifera laccase and Polyporus versicolor laccase in several perturbed states are reported. Coordination of fluoride to the …
A resonance Raman investigation of perturbed states of tree and fungal laccase Read more »
Sunney I. Chan, Craig T. Martin, Hsin Wang, Gary W. Brudvig, Tom H. Stevens, The Coordination Chemistry of Metalloenzymes, 313-328, 1983 Progress toward elucidation of the structure of the metal centers in cytochrome c oxidase will be reviewed. Our studies are based …
The structure of the metal centers in cytochrome c oxidase Read more »
Tom H. Stevens, Craig T. Martin, Hsin Wang, Gary W. Brudvig, Charles P. Scholes, Sunney I. Chan, Journal of Biological Chemistry 257, 12106-12113, 1982 The isolation and purification of yeast cytochrome c oxidase is described. Characterization of the purified protein …
Chan, Sunney I; Brudvig, Gary W; Martin, Craig T; Stevens, Tom H; in: "Electron Transport and Oxygen Utilization" (C. Ho, ed.) Elsevier Publishing Co., Amsterdam Electron Transport and Oxygen Utilization 171-177 1982
David F. Blair, Craig T. Martin, Jeff Gelles, Hsin Wang, Gary W. Brudvig, Tom H. Stevens, Sunney I. Chan, Chemica Scripta 21, 43-53, 1981 Studies directed toward the elucidation of the structures of the metal centers in cytochrome c oxidase …
METAL CENTERS OF CYTOCHROME c OXIDASE: STRUCTURES AND INTERACTIONS Read more »
Craig T. Martin, Randall H. Morse, Robert M. Kanne, Harry B. Gray, Bo G. Malmström, Sunney I. Chan, Biochemistry 20, 5147-5155, 1981 The reactions of nitric oxide (NO) with the oxi- dized and reduced forms of fungal and tree laccase, as well as with tree laccase depleted in type 2 copper, are reported. The products of the reactions were determined by NMR and mass spectroscopy, whereas the oxidation states of the enzymes were monitored by EPR and optical spectroscopy. All three copper sites in fungal lacease are reduced by NO. In addition,NO forms a specific complex with the reduced type 2 copper. NO similarly reduces all of the copper sites in tree lacease, but it also oxidizes the reduced sites produced by ascorbate or NO reduction. A catalytic cycle is set up in which N20, …
Reactions of nitric oxide with tree and fungal laccase Read more »