Elvan Cavac, Luis E. Ramírez-Tapia, and Craig T. Martin, J Biol Chem, 297(3), xxxx-yyyy, 2021. doi: 10.1016/j.jbc.2021.100999 High yields of RNA are routinely prepared following the two-step approach of high-yield in vitro transcription using T7 RNA polymerase followed by extensive purification using gel separation …

High salt transcription of DNA co-tethered with T7 RNA polymerase to beads generates increased yields of highly pure RNA Read more »

Yasaman Gholamalipour, William C. Johnson, & Craig T. Martin, Nucl Acids Res, 47, e118 (7 pages). 2019 In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as …

Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase Read more »

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Craig T. Martin, Charles P. Scholes, Sunney I. Chan, Journal of Biological Chemistry 260, 2857-2861, 1985 A histidine auxotroph of Saccharomyces cerevisiae has been used to metabolically incorporate [1,3-15N2] histidine into yeast cytochrome c oxidase. Electron nuclear double resonance (ENDOR) …

The identification of histidine ligands to cytochrome a in cytochrome c oxidase Read more »

Robert R. Birge, Albert F. Lawrence, Thomas M. Cooper, Craig T. Martin, David F. Blair, Sunney I. Chan, Nonlinear Electrodynamics in Biological Systems, 107-120, 1984 The energy stored in the primary photochemical event of light adapted bacteriorhodopsin (~16 kcal mol-1) …

The Energetics and Molecular Dynamics of the Proton Pumping Photocycle in Bacteriorhodopsin Read more »

DF Blair, GW Campbell, V Lum, CT Martin, HB Gray, BG Malmström, Sunney I. Chan, Journal of Inorganic Biochemistry 19, 65-73 Resonance Raman (RR) spectra of Rhus vernicifera laccase and Polyporus versicolor laccase in several perturbed states are reported. Coordination of fluoride to the …

A resonance Raman investigation of perturbed states of tree and fungal laccase Read more »

Craig T. Martin, Randall H. Morse, Robert M. Kanne, Harry B. Gray, Bo G. Malmström, Sunney I. Chan, Biochemistry 20, 5147-5155, 1981 The reactions of nitric oxide (NO) with the oxi- dized and reduced forms of fungal and tree laccase, as well as with tree laccase depleted in type 2 copper, are reported. The products of the reactions were determined by NMR and mass spectroscopy, whereas the oxidation states of the enzymes were monitored by EPR and optical spectroscopy. All three copper sites in fungal lacease are reduced by NO. In addition,NO forms a specific complex with the reduced type 2 copper. NO similarly reduces all of the copper sites in tree lacease, but it also oxidizes the reduced sites produced by ascorbate or NO reduction. A catalytic cycle is set up in which N20, …

Reactions of nitric oxide with tree and fungal laccase Read more »