in vitro - Martin Lab

A New Approach to RNA Synthesis: Immobilization of Stably and Functionally Co-Tethered Promoter DNA and T7 RNA Polymerase

By Martin Posted on July 1, 2024 Posted in Publications, Uncategorized Tagged with in vitro, RNA, rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

Kithmie MalagodaPathiranage, Ruptanu Banerjee, and Craig T. Martin, Nucleic Acids Research, July, 2024 Current approaches to RNA synthesis/manufacturing require substantial (and incomplete) purification post-synthesis. We have previously demonstrated the synthesis of RNA from a complex in which T7 RNA polymerase is …

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A Simple Approach to Improving RNA Synthesis: Salt Inhibition of RNA Rebinding Coupled With Strengthening Promoter Binding by a Targeted Gap in the DNA

By Martin Posted on July 7, 2023 Posted in Publications, Uncategorized Tagged with in vitro, rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, 691, 209-222, 2023 T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large amounts of RNA. Such synthesis, however, is often plagued …

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Efficient inhibition of RNA self-primed extension by addition of competing 3’-capture DNA – improved RNA synthesis by T7 RNA polymerase

By Martin Posted on August 8, 2019 Posted in Publications Tagged with in vitro, RNA, rna polymerase, RNA synthesis, T7 RNA polymerase, transcription

Yasaman Gholamalipour, William C. Johnson, & Craig T. Martin, Nucl Acids Res, 47, e118 (7 pages). 2019 In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as …

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3’ end additions by T7 RNA polymerase are RNA self-templated, distributive, and diverse in character – RNA-Seq analyses

By Posted on September 28, 2018 Posted in Publications Tagged with in vitro, RNASeq, T7 RNA polymerase, transcription

Yasaman Gholamalipour, Aruni Karunanayake Mudiyanselage, & Craig T. Martin, Nucl Acids Res, 46, 9253-9263, 2018. Selected by the editors as a “Breakthrough Article.” Synthetic RNA is widely used in basic science, nanotechnology and therapeutics research. The vast majority of this RNA is …

3’ end additions by T7 RNA polymerase are RNA self-templated, distributive, and diverse in character – RNA-Seq analyses Read more »

Insights into the Mechanism of Initial Transcription in E coli RNA Polymerase. Energetic Mechanisms Leading to Initial Complex Instability and Abortive Cycling

By Posted on September 18, 2013 Posted in Publications Tagged with abortive cycling, bacterial rna polymerase, in vitro, rna polymerase, T7 RNA polymerase, transcription

Satamita Samanta & Craig T. Martin, J. Biol. Chem. 288, 31993-32003, 2013. It has long been known that during initial transcription of the first 8-10 bases of RNA, complexes are relatively unstable, leading to the release of short abortive RNA transcripts. An …

Insights into the Mechanism of Initial Transcription in E coli RNA Polymerase. Energetic Mechanisms Leading to Initial Complex Instability and Abortive Cycling Read more »

New Insights into the Mechanism of Initial Transcription: the T7 RNA Polymerase Mutant P266L Transitions to Elongation at Longer RNA Lengths than Wild Type

By Posted on May 30, 2012 Posted in Publications Tagged with abortive cycling, in vitro, P266L, rna polymerase, T7 RNA polymerase, transcription

Luis Ramírez-Tapia & Craig T. Martin, J. Biol. Chem. 287, 37352-37361, 2012. RNA polymerases undergo substantial structural and functional changes in transitioning from sequence-specific initial transcription to stable and relatively sequence-independent elongation. Initially transcribing complexes are characteristically unstable, yielding short abortive products …

New Insights into the Mechanism of Initial Transcription: the T7 RNA Polymerase Mutant P266L Transitions to Elongation at Longer RNA Lengths than Wild Type Read more »

Direct Tests of the Energetic Basis of Abortive Cycling in Transcription

By Posted on May 30, 2011 Posted in Publications Tagged with abortive cycling, in vitro, initial transcription, initiation, rna polymerase, T7 RNA polymerase, transcription, transcription initiation

Ankit V. Vahia & Craig T. Martin, Biochemistry 50, 7015-7022, 2011. Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that …

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Tag: in vitro