Purnima Mala, Ruptanu Banerjee, Amin Abek, James Forster III, Aniruddha Pinjari, Ashish A. Kulkarni, and Craig T. Martin, Nucleic Acids Res, in press, 2025. This work aims to improve RNA synthesis and manufacturing, exemplified by T7 RNA polymerase-driven in vitrotranscription. We …
Kithmie MalagodaPathiranage, Ruptanu Banerjee, and Craig T. Martin, Nucleic Acids Research, 52(17), 10607-10618. 2024 Current approaches to RNA synthesis/manufacturing require substantial (and incomplete) purification post-synthesis. We have previously demonstrated the synthesis of RNA from a complex in which T7 RNA polymerase …
Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, 691, 209-222, 2023 T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large amounts of RNA. Such synthesis, however, is often plagued …
Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, 51(6), e36 (12 pages) T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science to …
Yasaman Gholamalipour, William C. Johnson, & Craig T. Martin, Nucl Acids Res, 47, e118 (7 pages). 2019 In vitro synthesized RNA is used widely in studies of RNA biology, biotechnology and RNA therapeutics. However, in vitro synthesized RNA often contains impurities, such as …
Yasaman Gholamalipour, Aruni Karunanayake Mudiyanselage, & Craig T. Martin, Nucl Acids Res, 46, 9253-9263, 2018. Selected by the editors as a “Breakthrough Article.” Synthetic RNA is widely used in basic science, nanotechnology and therapeutics research. The vast majority of this RNA is …
Satamita Samanta & Craig T. Martin, J. Biol. Chem. 288, 31993-32003, 2013. It has long been known that during initial transcription of the first 8-10 bases of RNA, complexes are relatively unstable, leading to the release of short abortive RNA transcripts. An …
Luis Ramírez-Tapia & Craig T. Martin, J. Biol. Chem. 287, 37352-37361, 2012. RNA polymerases undergo substantial structural and functional changes in transitioning from sequence-specific initial transcription to stable and relatively sequence-independent elongation. Initially transcribing complexes are characteristically unstable, yielding short abortive products …
Ankit V. Vahia & Craig T. Martin, Biochemistry 50, 7015-7022, 2011. Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that …
Direct Tests of the Energetic Basis of Abortive Cycling in Transcription Read more »
Xiaoqing Liu & Craig T. Martin, J. Biol. Chem. 284, 36262-36270, 2009. Transcription machinery from a variety of organisms shows striking mechanistic similarity. Both multi- and single subunit RNA polymerases have evolved an 8-10 base pair RNA:DNA hybrid as a part of …
Transcription Elongation Complex Stability: The Topological Lock Read more »