Purnima Mala, Ruptanu Banerjee, Amin Abek, James Forster III, Aniruddha Pinjari, Ashish A. Kulkarni, and Craig T. Martin, Nucleic Acids Res, in press, 2025. This work aims to improve RNA synthesis and manufacturing, exemplified by T7 RNA polymerase-driven in vitrotranscription. We …
Kithmie MalagodaPathiranage, Ruptanu Banerjee, and Craig T. Martin, Nucleic Acids Research, 52(17), 10607-10618. 2024 Current approaches to RNA synthesis/manufacturing require substantial (and incomplete) purification post-synthesis. We have previously demonstrated the synthesis of RNA from a complex in which T7 RNA polymerase …
Kithmie MalagodaPathiranage and Craig T. Martin, Methods in Enzymology, 691, 209-222, 2023 T7 RNA polymerase is widely used to synthesize RNA of any length, and long-standing protocols exist to efficiently generate large amounts of RNA. Such synthesis, however, is often plagued …
Kithmie MalagodaPathiranage, Elvan Cavac, Tien-Hao Chen, Bijoyita Roy, Craig T Martin Nucleic Acids Research, 51(6), e36 (12 pages) T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science to …
Craig T. Martin and Joseph E. Coleman, Biochemistry 26, 2690-2696, 1987 Specific interactions between T7 RNA polymerase and its promoter have been studied by asimple steady-state kinetic assay using synthetic oligonucleotide promoters that produce a short five-basemessage. A series of …