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RNA – Opportunities & Challenges
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T7 RNA Polymerase
Quick Intro
Transcription - a general overview
Mechanism and Structure
Mechanism and Structure
Promoter Binding
Elongation complex stability
Interactive structures
Structure - Initiation
Structure - initially transcribing
Structure - elongation
T7 RNA polymerase family
Practical Thoughts
Transcription simulation – gel analysis
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RNASq
RNASq – Seq Analysis Tools
Set up exptparams
RNASq Quick Start
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R for Biophysical Chemists
R – fitting data to a mathematical model
Phosphoramidite Synthesis Cycle
Quantifying gels
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T7 RNA polymerase - de novo
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